Research & Development

Bioregeneration at SAM: Experiment #4

EXPERIMENT #4
Seeds prepping – September 2th
Seeds sowing – September 3th
Seedlings Transplant – September 22nd
CO2 injection and Experiment Start – September 23rd
Matthias Ingress – October 13th
Plants harvest and kill – October 20th
WBA Ends – October 26th
TM cleaning and prep – October 27th to October 30th

This experiment coincides with the World’s Biggest Analog (WBA) and introduced a human in-the-loop.

By |2025-09-25T05:53:13+00:00September 2nd, 2025|Categories: Research & Development|0 Comments

Bioregeneration at SAM: a summary

Dwarf peas growing in the Test Module at SAM, at Biosphere 2.

Luna Powell and Atila Meszaros at SAM, following the first, complete configuration of an experiment in bioregeneration.

This spring brought us to the close of four years of design, development, and construction of a unique, world-class, hermetically sealed research facility for bioregeneration (air revitalization with plants) for long-duration human space exploration.

SAM is now actively hosting what will be the first of many years of experiments in which we grow a specific species of food cultivar to determine it’s growth rate and carbon sequestration against three Vapor Pressure Deficit values.

My graduate student Atila Meszaros and SAM Site Manager Luna Powell managed a perfectly executed test run of dwarf wheat, peas, and quinoa. The peas are a unique variety developed by Dr. Bruce Bugbee and his team at Utah State University. Smaller than standard, they produce a remarkably high seed yield–perfect for the Moon or Mars.

Furthermore, we are working with Dr. Lucie Poulet and her graduate student to determine key characteristics of the peas, including the density and dimensions of the leaves. All of this work is inspired by the legacy of NASA veteran Raymond Wheeler who was instrumental in motivating my dive into the world of bioregeneration in 2017 with my very first concept for SIMOC.

June through mid August saw completion of two full, six week runs of peas (2 weeks incubation at B2; 4 weeks in SAM) only to be met with the reality of one too many system failures and mistakes, forcing a total restart this past Thursday. That is the rigor of science–if the experiment is not done right, you do it again … and again. I am proud of Atila and Luna for not hesitating to set aside more than three months of hard, daily work in the name of solid science.

A dwarf variety of pea at SAM, Biosphere 2.

Our current experimental configuration is built around a nearly fully automated, computer controlled system that Atila, Luna, and Tanner Conrad, Research Technologist (under Dr. Murat Kacira) at UA CEAC assembled and programmed. It maintains a constant 800 ppm of CO2 such that by monitoring the amount of CO2 injected (from a cryogenic tank via gas manifold) we know the maturity of the peas and can plot, with a high degree of accuracy, the growth function and as such, ability for any food cultivar to revitalize cabin air.

The culmination of this series of experiments comes in October in conjunction with the World’s Biggest Analog where veteran SAM team member Matthias Beach will be sealed inside for two weeks. During the first week his CO2 will be sequestered by the peas (at least, that’s the hypothesis). On the morning of the 8th day he will harvest the peas, secure them in air-tight bags, and then complete the mission with no CO2 removal to demonstrate the amount of CO2 that was being sequestered by the plants.

Learn more about this series of experiments …

By |2025-09-25T18:47:37+00:00August 23rd, 2025|Categories: Research & Development|0 Comments

A summer of adventure

This summer was one of adventure for the entire SAM crew.

Kai and Trent enjoyed a trip down the Grand Canyon mid-May with directors and volunteers for the National Space Society, the final voyage of this seasonal journey in memory of the incredible (and greatly missed) Anita Gale who departed planet Earth in May 2024.

Kai and Colleen attended the National Geographic Society’s Explorers Fest and then ventured on to Mongolia for six weeks, teaching English, exploring the foot of the country’s largest glacier, and kayaking wild rivers.

Kai Nevers and his partner Kate spent a month traveling around Greece and Italy … ending the trip with a 1 week hut-to-hut hike in the Dolomites.

Trent was wreck diving with the Explorers Club in the Great Salt Lakes.

Luna enjoyed time with family in rural Maine and sought refuge from the summer heat in Northern Arizona.

Griffin took his first trip overseas and presented two papers for SAM at ICES 2025, Prague.

Atila explored the beaches and jungle of his home country Peru.

Bindhu attended the Humans to the Moon and Mars summit in Washington D.C. followed by the Aerospace Medical Association conference in Atlanta, Georgia, related to the SAM MedBay project. She visited a colleague from the Analog Astronaut Conference in England, where she rode her first wave on a surf board, visited the Eden Project, and prehistoric Stonehenge.

Nathan explored lava tubes in Hawaii.

Shantano got his first, single author paper accepted to the CAIP conference, presented at the Sagan Summer Workshop for a hands-on project on occurence rate of exoplanets, and captured a thunderstorm and Saturn’s moon Titan on his phone.

And Matthias ventured to Devon Island with the Mars Society as XO for the Advance Team to prepare the Flashline station for Missions 17 and 18.

And somehow, despite the incredible travel, we got a lot done at SAM!

By |2025-09-26T15:29:18+00:00August 1st, 2025|Categories: Research & Development|0 Comments

Bioregeneration at SAM: mid first run

The following was written by University of Arizona graduate student Atila Meszaros, and lead researcher on Bioregeneration experiments at SAM. Any changes from the original body of text are in [brackets].

We are reaching the end of the second week of our first peas-experiment, still tweaking here and there, but learning pretty much every day. We are developing protocols and knowledge that will help us perfect growing cultivars at SAM for the next few years.

Since the last TODO list, before the experiment, we had 43 different activities to complete, each one with its own ramifications and tasks. But with everyone’s help we managed to pull through and we were able to finish the set up for the experiment. The next runs should be relatively painless from now on [in theory].

  • All monitoring and control for our main systems are wired, programmed, automated, and up and running. Something I will tackle soon, for both my thesis and SAM, is [a compilation of] the physical and computing processes—how I wired everything to each line of code, [including] video tutorials on how to operate Campbell and Logger net.
  • All data is saved in several places, the local computer at the IT room, [our shared SAM] Google Drive, and my personal PC. We are taking measurements every second, I will probably change it to every 10 seconds if the data gets too heavy to process. Just in 10 days we had 8 million data points. A little bit too much but it doesn’t hurt for now.
  • All racks are fully functional and with newly installed devices: extra lights, extra fans, new pumps, water pressure probes. We are reaching around 500 PPFD on our racks. Comparing it with Dr. Wheeler’s and Chinese Lunar Palace, their numbers vary between 400-600 for their highest output crops, and 700 PPFD for wheat. We are in a good range for our PPFD, although I would like to implement the dimmers in the future to have more flexibility. However, this also means that more lights cannot be added unless they are intercanopy lights that go in between the plants.
  • Temperature as we know is our biggest issue now. Hopefully with the recharge of the [failed mini-split unit], this gets solved for the foreseeable future. Our brand-new humidifier system is working perfectly, providing consistent values between the 40-45% relative humidity. We don’t see any reason why it would not provide the same consistency on higher humidities as we move the VPD. All the Whirlpool dehumidifiers have a range between 35%-80% currently setting is 45%. We haven’t seen any algae accumulation in the translucent tubes.
  • The crops seem to be growing strong and healthy. Revisiting the reflective wall experiment, where it took 20 days for us to see the first pea pods, 12 days since the transplanting, we are already seeing some of them in all the racks. One disadvantage of not being able to go inside, is not being able to record events like these every day. The addition of inner-rack cameras could be a possibility that we add in the future.
  • The CO2 injection gave us some problems the first days, as we were figuring out how to properly manage the regulator + CO2 tank. The CO2 tank gauge needs to be completely open, while every pressure change is in the regulator. I know it makes sense as I said it, but we didn’t want to have too much pressure at the beginning, so we half-opened the CO2 tank gauge. [Then] we find out that that CO2 gas output would shut off. Now, the system is working as intended, and we have consistent CO2 injections.
  • Now, on CO2. After some comparison between the SIMOC arrays, Campbell, and the handheld CO2 device. We have concluded that the SIMOC values at the TM are off by approximately 100 ppm. Talking with Ezio he mentioned that the calibration might be off. The numbers are ultimately constant, with the same offset value at different points. I am confident we can just process this post data recollection. I didn’t want to change the offset just yet, in case I was wrong, and it was an error from my devices.
  • The CO2 addition during the time that we go inside the TM is considerable. 20 minutes between Luna and I can increase more than 100 ppm. Every time we go inside, we have a specified agenda that we tackle as fast as possible. Tomorrow, we have a scheduled ingress, and I am thinking about running the blower with extra ports open so when we worked inside the CO2 ppm remains closer to 800, instead of taking longer to stabilize.
By |2025-09-25T18:46:48+00:00June 26th, 2025|Categories: Research & Development|0 Comments

New SAM Team page

SAM Team montage

Since January 2021 the SAM team has grown from Kai Staats and Trent Tresch and a host of volunteers to an international cadre of staff members who contribute a wealth of knowledge, experience, skills, and motivation to bring to life an advanced research center for human space exploration.

Visit the all-new SAM Team page

By |2025-05-05T18:13:20+00:00April 25th, 2025|Categories: Research & Development|0 Comments

Welcome Griffin Hentzen!

This year will see a shift in the SAM team. While in a corporate environment it is expected that the team and total productivity always grow, in an academic environment teams fluctuate—semester to semester, research project to research project, year to year.

At the start of the SAM project in January 2021 all team members were volunteers, including Kai and Trent. With a dozen volunteers that spring, the team then shrunk to just a few in the fall, growing steadily again through 2023. Volunteers provided what time they had. Some became paid staff. Students graduated and moved to jobs in their field.

The fall of 2024 was a transition with the realization that the SAM project had matured, now requiring more than pairs of willing hands and a willingness to learn new skills—SAM needs focused skill-sets and experience to bring specific ideas to form. This resulted in our first ever job posting and a new hire.

Griffin Hentzen comes to us from Purdue where he recently graduate with a BSc in Aerospace Engineering from Purdue University. He has interned at Sierra Space for two semesters, with a focus in carbon dioxide scrubber systems. He will be focusing this year on the design and fabrication our new CO2 scrubber at SAM, working closely with Dr. James Knox (also a part of the SAM team) and Director of Research lead Kai Staats, while lending a hand in myriad tasks as presented.

Welcome Griffin!

By |2025-01-30T06:07:28+00:00January 15th, 2025|Categories: Research & Development|0 Comments

With the close of 2024

The SAM Team has this fall sustained a rigorous forward progress. Unlike the prior three years of design, development, and fabrication, this semester has seen us developing programs and collaborations as much as physical structures. This growth is welcomed, but it has also broken the tradition of weekly updates in the form of photo essays to this blog site.

As such, until those stories can be built, backdated, and posted, here is a quick summary.

  • We have returned to the process of leak detection and patching in order to extend the time SAM can remain pressurized without adding air, for those missions that desire to operate in Mode 3.
  • Dr. Sean Gellenbeck, Luna Powell and Matthias Beach, have moved into the construction of an automated hydroponics nutrient management and delivery and light control for the SAM hydroponics system. The Campbell Scientific system is now installed, with programming slated for late January into February. Project leadership transitions to graduate student Atila Meszaros with the start of this spring 2025 semester.
  • A new CO2 scrubber research facility is being constructed between the Crew Quarters and the Air Intake Room (SAM AIR) to close the loop. To be built on a licensed NASA patent with NASA veteran Dr. James Knox as the model lead (since February 2024) for our team.
  • The SIMOC Live team continues to evolve and improve this portable, ad hoc network air quality monitoring and model validation system built on open source software and the combination of Raspberry Pi computers and Adafruit sensor boards.
  • The Mars yard gravity offset rig is being rebuilt from welded aluminum with a transition from static climbing rope to braided steel cable for the suspended counter weight system for a tighter, more static and responsive system with less inertial lab.
  • During the months of November and December Dr. Cameron Smith and Kai Nevers with assistance from Trent Tresch, Ivy Wahome, and Matthias Beach designed, fabricated, and tested an emergency inflatable shelter for Mars. This functional prototype is now housed at SAM with a handful of potential patents already in motion. Photographs and blog entries will be posted as soon as the IP is more fully defined. Stay tuned!
  • Dr. Bindhu Oommen and Kai Staats, MSc are leading the design, development, and fabrication of an full-featured surgical bay for installation at SAM. This future-looking project begins with a systematic, mathematical analysis of the urgency of a medical emergency, the capabilities of the surgical bay, and the distance from a more advanced facility if transport is required for the ultimate procedure. This project is now several months in motion, with a first-ever workshop held at Biosphere 2 and SAM January 17-19 with eight world-class surgeons joining in person to take this concept to the next level.
  • Completing the remodel of Ops which was set back by a semi-major water flood this summer. Principal focus is on a fully functional kitchen, updates to the Mission Control Center, and hanging several prints to add a little color to the rooms.
  • Phase I of a multi-year, multi-year research project, the very project for which SAM was built, begins as soon as the IRB approval is complete. This will see ten of the SAM team members each staying inside of SAM for 58 hours in order to monitor CO2 level rise in the sealed facility with no CO2 scrubber and no plants. Body mass, food intake, and activities will be recorded.

And that is just the beginning of what will prove to be the most exciting phase of developments at SAM in 2025!

By |2025-01-15T07:22:30+00:00January 6th, 2025|Categories: Construction, Research & Development|0 Comments
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