kai

It is our pleasure to bring to a close Phase I development and construction of a Space Analog for the Moon and Mars (SAM) at Biosphere 2. This past six months of research, design, engineering, and hands-on construction has been a tremendous labor of passion by Kai Staats, Trent Tresch, Biosphere 2 Deputy Director John Adams, Tim Mcmullen and Terry Murchek of Biosphere 2’s maintenance staff, intern Natasha Loving, and our volunteers Cameron Smith, KC Shasteen and Michael Blum, Linnaea Groh and Atila Meszaros, Robert David and Angus Gluck, Colleen Cooley, Trenton Kenney, and Jolene Varga and Rob Ronci.

We thank Executive Director Joaquin Ruiz and Deputy Director of Research Cherry Murray and the whole of the Biosphere 2 staff for continued, daily support; Murat Kacira and Gene Giacomelli of the University of Arizona Controlled Environment Agriculture Center; and Doug Hocksteed and Rakhi Gibbons of TechLaunch Arizona for the initial round of funding. Jude Yandow was instrumental in keeping the finances in order and Julie Stringer for helping us navigate the complex framework of the University.

We look forward to returning in September to dive back into SAM construction, with the first of three shipping containers already in place for the 1200 sq-ft crew living quarters.

See you then!

We did it! We completed the first seal of the fully refurbished Test Module at Biosphere 2! The experience was extraordinary, a true celebration of the effort to bring this iconic prototype pressure vessel back to life!

The day started with long-time B2 electrician Kevin installing a new 100A, 3-phase disconnect in our primary panel at SAM. Then Chris Kaufmann, Brian Scott, Neal Barto, Emma Menden, and Michael Mason from University of Arizona CEAC delivered, assembled, and transplanted a full suite of food cultivars (some 180 in all), the first to arrive to SAM. Trent moved the CO2 scrubber into place while John and Kai established a base-line for the sensor array, both internal to and external to the sealed Test Module. Leonardo Buono, a veteran filmmaker was on-site for the entire day to both film and manage the lung inflation fan and valve.

With a few words spoken prior to entry, the crew walked inside and sealed the door.

Kai Staats, Trent Tresch, John Adams, Katie Morgan, and reporter Jessica Camille Aguirre were sealed inside for four and a quarter hours. While monitoring the CO2, O2, temperature, relative humidity, and barometric pressure, they played Xtranaut, a board game developed by Dr. Dante Lauretta at the University of Arizona, Principal Investigator for OSIRIS-REx, the spacecraft that is returning a sample from the carbonaceous asteroid Bennu. Finally, this first team to enter the fully refurbished Test Module assembled a FarmBot, an open source precision agriculture CNC farming tool. Our unit was donated to SAM by founder Rory Aronson. It is beautifully constructed, an elegant machine we are eager to employ later this year.

This marks the first time in 30 years that humans have been sealed inside the Test Module and the completion of Phase I construction of SAM, a Space Analog for the Moon and Mars at Biosphere 2.

We were honored to have colleagues and family waiting outside SAM for the duration of the test run, greeting us as we exited at 8:30 pm with a bottle of bubbly and lemonade. Thank you for your support, and for keeping Leo company!

Now, we have data to analyze, photos and video to process, and six months of work to bring to a close.

Test Module Dry Run | Five Persons Sealed Inside | Data Analysis

Following six months demolition, construction, and revitalization of the prototype for the Biosphere 2, the Test Module is sealed and pressurized for the first time in 30 years! Kai Staats, Trent Tresch, and Biosphere 2 Deputy Director John Adams discuss this important endeavor and then activate the blower which causes the lung to inflate and the massive pan to rise, providing a hermetically sealed space within the Test Module.

Test Module Dry Run | Five Persons Sealed Inside | Data Analysis

Diagram of the Test Module and Lung, circa 1987

The Test Module lung was developed as a “method of managing the effects an internal temperature and external barometric pressure change could cause in a fixed, sealed, glass structure. This problem was solved with a variable volume system joined to the module by an air duct. With increased temperature or decreased barometric pressure in the Test Module compared to the out- side environment, the variable chamber expands; with a decrease in temperature or a increase in pressure, the chamber contracts. The lung structure provides an effective means to prevent the possibility that the Test Module would implode or explode when subjected to these forces. The reservoir of air provided an increased buffering; adding approximately 20-40% to the total atmospheric volume. The weight of the pan on the lung structure insured a positive displacement from inside the closed system to the outside.” — Abigail Alling, Linda Leigh, Taber MacCallum, and Norberto Alvarez-Romo. Biosphere 2 test module experimentation program. Biological Life Support Systems 23 (1990): 32.

This week was the last, big push for resealing the Test Module and lung—dozens of small details and a few substantial undertakings before our first pressure tests on Monday.

There are 18 points at which the Test Module pressure vessel is penetrated, including the entrance and a 7-port gas exchange manifold. In the 1987-89 test runs these served various purposes: monitoring the internal air and water (drinking, waste, marsh); moving sensor data over physical cables; exchanging hot and cold water for the heat exchanger then mounted in the overhead space frame. The clean water inlet will be reainted, but used sparingly (everything that goes in, stays in). The 3″ diameter copper feeds for the heat exchanger are terminated and capped. Since the ’80s much has changed in data transmission. Now a single wireless feed can readily transmit a vast quantity of real-time data and video. However, two hard line connections (Ethernet, USB) will be installed in order to rebuild or update computers and embedded devices (e.g. WiFi router), and as a back up should the wireless go down.

As such, we have reduced the number of ports to:

• A fresh water inlet
• Wired data (Ethernet + USB)
• Electrical feeds from the external, primary panel to the internal sub-panel
• Continued use of the original, 7-port gas exchange manifold
• Two new manifolds for the mini-split heat pumps (coolant, power)

The lung is an adjacent structure connected to the Test Module by means of a 100 cubic foot, underground corridor (tube) large enough to crawl through. The lung is composed of a concrete structural frame and welded steel, cylindrical wall and floor. The upper lip of the cylinder is sealed to the larger end of the flexible rubber membrane (not unlike the rubber used on inflatable river rafts) from which is suspended a 22 foot diameter steel pan which itself is attached to the lower end of the membrane, and re-sealed (per our effort today). The area above the pan is open to the outside air. The area below the pan is an extension of the Test Module volume itself, with air movement through the restricted corridor.

The lung also has more than 20 penetrations. All but five were capped or plugged. As with the Test Module, all unused ports in the lung are sealed with Teflon tape over threaded plugs or caps.

As such, the functional ports are:

• A single feed gas manifold (may or may not be retained)
• Lung inflation fan with 3″ ball valve
• Electrical feed to lights
• Water column pressure release “P” trap

The inflated lung provides a positive pressure internal to the living space, an automated compensation for both internal temperature changes and external barometric pressure changes, and a buffer such that for the duration of a simulation, a hermetic seal may be maintained. The better the total seal, the longer a simulation can run without adding outside air.

When the Test Module and lung were inspected in the fall of 2020, it was clear that a great deal of work would be needed to regain a fully sealed function. The curved steel ring segments that held the rubber membrane in place were completely rusted from thirty years of water and weather, to the point that many of the threaded rods simply snapped off or turned to dust in one’s fingers.

Kai, Trent, and a host of volunteers have worked on various aspects of lung repair since January ’21, focused on patching the membrane itself, restoring the surface of the lower ring, welding 220 new threaded rods (studs), sealing against further rust, and then, finally, restoring the seal with a new set of individual 4″ stainless steel plates, replacing the nine heavily rusted angle iron rings segments. Furthermore, the outer shell of the lung has been completely sealed with silicone caulk and a 100% elastomeric such that only in the worst storms might a small amount of wind driven rain find its way onto the upper, external facing side of the membrane.

As of the posting of this photo essay, the silicone is a half dozen hours into a 72 hours cure. We are confident that we have a solid seal between the membrane and the lower ring of the steel pan. With the lower lung door rebuilt and ready to be installed there are no known open ports or holes in the entire pressure vessel. However, we remain aware that one or more holes may exist and that no system is fully sealed.

Our fingers are crossed for success in pressurizing the Test Module for the first time in thirty years!

Stay tuned!

The Test Module lung was developed as a “method of managing the effects an internal temperature and external barometric pressure change could cause in a fixed, sealed, glass structure. This problem was solved with a variable volume system joined to the module by an air duct. With increased temperature or decreased barometric pressure in the Test Module compared to the out- side environment, the variable chamber expands; with a decrease in temperature or a increase in pressure, the chamber contracts. The lung structure provides an effective means to prevent the possibility that the Test Module would implode or explode when subjected to these forces. The reservoir of air provided an increased buffering; adding approximately 20-40% to the total atmospheric volume. The weight of the pan on the lung structure insured a positive displacement from inside the closed system to the outside.” — Abigail Alling, Linda Leigh, Taber MacCallum, and Norberto Alvarez-Romo. Biosphere 2 test module experimentation program. Biological Life Support Systems 23 (1990): 32.

The “lung” is a pressure regulation and air storage system first tested 33 years ago as part of the Test Module program. It was then improved upon and scaled to a much larger volume for the Biosphere 2 proper. Today Trent Tresch and volunteer Rob Ronci of Colorado were successful in conducting a dry run inflation of the refurbished Test Module lung.

With the lower lung door only partially sealed, the electrical sub-panel ports not yet complete, and one known leak in the Test Module space frame structure, the lung membrane inflated and rose to an inverted position in just a few minutes of running the inflation fan.

This bodes well for what we believe will be a fully functional test of the lung early next week.

We are just ten days from the conclusion of Phase I of construction of SAM, and a week from the start of a series of pressurized tests in which we will monitor temperature, humidity, CO2, O2, and both interior and exterior atmospheric pressures as we seal the Test Module for varied durations of time.

June 20 also marks five months labor at the SAM analog site, from the early efforts in pushing back the desert growth to stripping the Test Module interior down to the frame and grinding, sanding, and cleaning the lung pan, ring, and membrane. In putting it all together again we have primed and painted, welded and wired, shoveled, cut, caulked, sealed, glued and cemented from sunrise to sunset, four to five days a week since January 20.

This kind of adventure may not take its participants across high seas or through dense jungles, but the arduous effort is rewarding in a similar manner. A passion for achieving difficult goals, attention to detail, problem solving, and working within a highly capable, agile team. Our race to the finish is not one of competition with others, but one of upholding a pledge to ourselves, partners, and investor Tech Launch Arizona that we will by the close of the University of Arizona fiscal year refurbish and revive the 1987 Biosphere 2 prototype Test Module as the cornerstone of SAM.

This is our list of actions items that remain, with one week to complete them all:

• Install two 2 ton mini-split heat pumps (air conditioning).
• Complete reconstruction of the sealed, lower lung door.
• Apply the remaining patches to the lung membrane.
• Patch the one known exterior break in the original silicone sealant.
• Run electrical power from the exterior 100A service to the sub-panel; then
• Build-out four electrical 120V sockets for interior use; and
• Wire the overheads lights to a switch.
• Fully bleach and scrub the lung interior.
• Install the interior manual pressure release valve.
• Conduct a dry pressure test before re-attaching the lung membrane to the ring.

Thank you Sean Gellenbeck, aerospace engineer at Paragon Space Development Corporation and PhD student at the University of Arizona for stopping by SAM and lending an expert hand! Your experience with varied materials and rapid development was well received!